P-50: Effect of Antioxidants (E, C, Astaxanthin Vitamins) on Sperm Parameters with Restricted High Fat Diet in Rats

Background: Obesity increases the likelihood of various diseases, particularly heart disease, type 2 diabetes, obstructive sleep apnea, certain types of cancer and infertility and dub fertility. Some studies have associated the body mass index (BMI) with reproductive parameters in men, showing that increased BMI is related to poor semen quality, decreased sperm concentration, decreased normal-motile sperm cells and increased DNA fragmentation index. In this study we studied the effects of Antioxidants (E, C, Astaxanthin vitamins) on sperm parameters with restricted high fat diet in rats. Materials and Methods: Matured wistar albino male rats weighting approximately 200 - 250 g were divided to 3 groups; control with access to standard diet (group I), group with high-fat diet (group II) and animals received high-fat diet and Antioxidants such as vitamins E and C, Astaxanthin (group III). After at three months, the rats were sacrificed and sperm parameters such as sperm count, motility, morphology and viability were analyzed. Results: The mean values of sperm parameters showed that progressive motility of spermatozoa in group II and III (13% and 16.81%, respectively) were significant lower than group I (35.36%) as well as normal morphology in group II and III (95.11% and 93.3%, respectively) was decreased rather than group I (99%). But there were no significant difference between sperm count and viability of three groups. Conclusion: The present results suggest that restricted high fat diet can lead to reduction sperm motility and normal morphology, but treatment with antioxidants such as E, C and Astaxanthin vitamins could not compensated and improvement this deficiency.